Gelatinase and the Gates-gilman-cowgill Method of Pepsin Estimation
نویسنده
چکیده
The Gates photographic film method for pepsin estimation as developed by Gilman and Cowgill measures an activity corresponding to that determined by the hemoglobin method of Anson and Mirsky rather than that resulting from the use of the gelatin viscosity technique. Therefore, the presence of gelatinase is not a source of great error in the gelatin film procedure.
منابع مشابه
The gastric secretion of pepsin in man TABLE III
The control of gastric pepsin secretion continues to excite controversy. The diversity in the results obtained by different investigators may be due, at least in part, to the varying techniques employed to estimate pepsin activity. Thus, edestin (Polland and Bloomfield, 1929), gelatin (Gilman and Cowgill, 1930), egg albumen (Vineberg and Babkin, 1931), beef haemoglobin (Anson and Mirsky, 1932),...
متن کاملThe gastric secretion of pepsin in man TABLE
The control of gastric pepsin secretion continues to excite controversy. The diversity in the results obtained by different investigators may be due, at least in part, to the varying techniques employed to estimate pepsin activity. Thus, edestin (Polland and Bloomfield, 1929), gelatin (Gilman and Cowgill, 1930), egg albumen (Vineberg and Babkin, 1931), beef haemoglobin (Anson and Mirsky, 1932),...
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Few articles on concentration of pepsin in the gastric juice of patients with disease of the stomach and duodenum are to be found in recent literature, and the results reported by different observers are not always in accord. We shall consider here only the concentration of pepsin in the gastric juice of persons suffering with the syndrome of peptic ulcer. Wilcox (5), in 1908, reported that the...
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Despit.e the numerous methods for the determination of proteolytic enzymes, there are few, if any, which incorporate all the advantages of accuracy, simplicity, and brevity, and which require only a small sample for a determination. In 1927 Gates (1) published a preliminary report of a proteolytic enzyme titration possessing these desirable features, and demonstrated his method at the 1928 meet...
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عنوان ژورنال:
- The Journal of General Physiology
دوره 17 شماره
صفحات -
تاریخ انتشار 2003